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excess nhs dpeg 12 biotin  (Vector Laboratories)


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    Vector Laboratories excess nhs dpeg 12 biotin
    Excess Nhs Dpeg 12 Biotin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/excess nhs dpeg 12 biotin/product/Vector Laboratories
    Average 93 stars, based on 6 article reviews
    excess nhs dpeg 12 biotin - by Bioz Stars, 2026-05
    93/100 stars

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    Overview of the experimental design. (A) Gelatin hydrogels were modified with NHS-dPEG 12 -biotin and loaded with avidin to investigate biotin-avidin-mediated crosslinking. (B) Gelatin polymers were biotinylated by reaction with NHS-dPEG 12 -biotin via conjugation to primary amines on gelatin. MarvinSketch was used for illustrating chemical structures, substructures and reactions, Marvin 20.15.0, 2020, ChemAxon ( http://www.chemaxon.com ). (C) Hydrogels were formed by allowing biotinylated gelatin polymers, prepared with 0.1 or 1 fold molar excesses (FME) of biotinylation reagent, to gelate overnight. Then, they were either placed directly into 17.1 or 85.5 μM avidin solutions for swelling studies in the presence of avidin or incubated overnight in 85.5 μM avidin prior to swelling studies in PBS to evaluate biotin-avidin stability.
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    nhs dpeg 12 biotin  (Vector Laboratories)
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    Vector Laboratories nhs dpeg 12 biotin
    Overview of the experimental design. (A) Gelatin hydrogels were modified with NHS-dPEG 12 -biotin and loaded with avidin to investigate biotin-avidin-mediated crosslinking. (B) Gelatin polymers were biotinylated by reaction with NHS-dPEG 12 -biotin via conjugation to primary amines on gelatin. MarvinSketch was used for illustrating chemical structures, substructures and reactions, Marvin 20.15.0, 2020, ChemAxon ( http://www.chemaxon.com ). (C) Hydrogels were formed by allowing biotinylated gelatin polymers, prepared with 0.1 or 1 fold molar excesses (FME) of biotinylation reagent, to gelate overnight. Then, they were either placed directly into 17.1 or 85.5 μM avidin solutions for swelling studies in the presence of avidin or incubated overnight in 85.5 μM avidin prior to swelling studies in PBS to evaluate biotin-avidin stability.
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    https://www.bioz.com/result/nhs dpeg 12 biotin/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
    nhs dpeg 12 biotin - by Bioz Stars, 2026-05
    93/100 stars
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    Overview of the experimental design. (A) Gelatin hydrogels were modified with NHS-dPEG 12 -biotin and loaded with avidin to investigate biotin-avidin-mediated crosslinking. (B) Gelatin polymers were biotinylated by reaction with NHS-dPEG 12 -biotin via conjugation to primary amines on gelatin. MarvinSketch was used for illustrating chemical structures, substructures and reactions, Marvin 20.15.0, 2020, ChemAxon ( http://www.chemaxon.com ). (C) Hydrogels were formed by allowing biotinylated gelatin polymers, prepared with 0.1 or 1 fold molar excesses (FME) of biotinylation reagent, to gelate overnight. Then, they were either placed directly into 17.1 or 85.5 μM avidin solutions for swelling studies in the presence of avidin or incubated overnight in 85.5 μM avidin prior to swelling studies in PBS to evaluate biotin-avidin stability.

    Journal: Frontiers in Chemistry

    Article Title: Effects of Biotin-Avidin Interactions on Hydrogel Swelling

    doi: 10.3389/fchem.2020.593422

    Figure Lengend Snippet: Overview of the experimental design. (A) Gelatin hydrogels were modified with NHS-dPEG 12 -biotin and loaded with avidin to investigate biotin-avidin-mediated crosslinking. (B) Gelatin polymers were biotinylated by reaction with NHS-dPEG 12 -biotin via conjugation to primary amines on gelatin. MarvinSketch was used for illustrating chemical structures, substructures and reactions, Marvin 20.15.0, 2020, ChemAxon ( http://www.chemaxon.com ). (C) Hydrogels were formed by allowing biotinylated gelatin polymers, prepared with 0.1 or 1 fold molar excesses (FME) of biotinylation reagent, to gelate overnight. Then, they were either placed directly into 17.1 or 85.5 μM avidin solutions for swelling studies in the presence of avidin or incubated overnight in 85.5 μM avidin prior to swelling studies in PBS to evaluate biotin-avidin stability.

    Article Snippet: After estimating the number of amine groups present as described above, a 25 mM stock solution of NHS-dPEG 12 -biotin was prepared in DMSO (Sigma-Aldrich).

    Techniques: Modification, Avidin-Biotin Assay, Conjugation Assay, Incubation

    Effects of biotin concentration on swelling properties in avidin swelling buffer. (A,B) Fold changes in swelling ratios compared to day 0 time point of hydrogels biotinylated with 0, 0.1, or 1 FME NHS-dPEG 12 -biotin in (A) 17.1 μM avidin or (B) 85.5 μM avidin for 7 days. n = 3, mean ± SD. Statistical significance between different FME hydrogels determined using two-way ANOVA with Tukey's post-hoc test (0 FME vs. 0.1 FME: ** p < 0.01, *** p < 0.001; 0 FME vs. 1 FME: + p < 0.05, ++ p < 0.01; 0.1 FME vs. 1 FME: # p < 0.05). (C–E) Fold changes in swelling ratios of hydrogels with (C) 0 FME, (D) 0.1 FME, or (E) 1 FME NHS-dPEG 12 -biotin, in either 17.1 or 85.5 μM avidin solutions, over 7 days. n = 3, mean ± SD. No statistical significance resulting from different avidin concentrations, determined using two-way ANOVA with Sidak's post-hoc test. Statistical significance between time points determined using two-way ANOVA with Tukey's post-hoc test ( % indicates p < 0.05 compared to the previous day).

    Journal: Frontiers in Chemistry

    Article Title: Effects of Biotin-Avidin Interactions on Hydrogel Swelling

    doi: 10.3389/fchem.2020.593422

    Figure Lengend Snippet: Effects of biotin concentration on swelling properties in avidin swelling buffer. (A,B) Fold changes in swelling ratios compared to day 0 time point of hydrogels biotinylated with 0, 0.1, or 1 FME NHS-dPEG 12 -biotin in (A) 17.1 μM avidin or (B) 85.5 μM avidin for 7 days. n = 3, mean ± SD. Statistical significance between different FME hydrogels determined using two-way ANOVA with Tukey's post-hoc test (0 FME vs. 0.1 FME: ** p < 0.01, *** p < 0.001; 0 FME vs. 1 FME: + p < 0.05, ++ p < 0.01; 0.1 FME vs. 1 FME: # p < 0.05). (C–E) Fold changes in swelling ratios of hydrogels with (C) 0 FME, (D) 0.1 FME, or (E) 1 FME NHS-dPEG 12 -biotin, in either 17.1 or 85.5 μM avidin solutions, over 7 days. n = 3, mean ± SD. No statistical significance resulting from different avidin concentrations, determined using two-way ANOVA with Sidak's post-hoc test. Statistical significance between time points determined using two-way ANOVA with Tukey's post-hoc test ( % indicates p < 0.05 compared to the previous day).

    Article Snippet: After estimating the number of amine groups present as described above, a 25 mM stock solution of NHS-dPEG 12 -biotin was prepared in DMSO (Sigma-Aldrich).

    Techniques: Concentration Assay, Avidin-Biotin Assay

    Journal: Frontiers in Chemistry

    Article Title: Effects of Biotin-Avidin Interactions on Hydrogel Swelling

    doi: 10.3389/fchem.2020.593422

    Figure Lengend Snippet: Theoretical avidin binding in biotinylated gelatin hydrogels, assuming 1:1, 1:2, 1:3, and 1:4 avidin to biotin binding.

    Article Snippet: After estimating the number of amine groups present as described above, a 25 mM stock solution of NHS-dPEG 12 -biotin was prepared in DMSO (Sigma-Aldrich).

    Techniques: Avidin-Biotin Assay, Binding Assay